Once cleaved, a propeptide generally has no independent biological i PRO_0000028353 This subsection of the PTM / Processing section describes a propeptide, which is a part of a protein that is cleaved during maturation or activation. This subsection of the 'PTM / Processing' section denotes the presence of an N-terminal signal peptide i This section describes post-translational modifications (PTMs) and/or processing / Processing i Molecule processing Feature key It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed lineage iĬellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus This subsection of the Names and taxonomy section contains the taxonomic hierarchical classification lineage of the source organism. This is known as the 'taxonomic identifier' or 'taxid'.More.Taxonomic identifier i This subsection of the Names and taxonomy section shows the unique identifier assigned by the NCBI to the source organism of the protein. This subsection of the Names and taxonomy section provides information on the name(s) of the organism that is the source of the protein i
More information in the GO evidence code guide Used to indicate a direct assay for the function, process or component indicated by the GO term.
a chemical reaction that the enzyme activity i This subsection of the Function section describes the catalytic activity of an enzyme, i.e.
Manual assertion inferred from sequence similarity to i Manually curated information which has been propagated from a related experimentally characterized protein. During oocyte activation, plays a role in cortical granule reaction in the zona reaction, which contributes to the block to polyspermy (By similarity). By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. **Derived using calibration standard 7-Amino, 4-Methyl Coumarin (AMC) (Sigma, Catalog # A9891).Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. Read at excitation and emission wavelengths of 380 nm and 460 nm (top read), respectively, in kinetic mode for 5 minutes.Īdjusted V max* (RFU/min) x Conversion Factor** (pmol/RFU).Include a Substrate Blank containing 50 µL Assay Buffer and 50 µL of 200 µM Substrate without any rhPLAT. Load 50 µL of the 2 ng/µL rhPLAT into a black well plate, and start the reaction by adding 50 µL of 200 µM Substrate.Dilute Substrate to 200 µM in Assay Buffer.Dilute rhPLAT to 2 ng/µL in Assay Buffer.Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent.F16 Black Maxisorp Plate (Nunc, Catalog # 475515).Fluorogenic Peptide Substrate Z-Gly-Gly-Arg-AMC (Bachem, Catalog # I-1140), 10 mM stock in DMSO.Recombinant Human t‑Plasminogen Activator/tPA (rhPLAT) (Catalog # 7449-SE).